Cleaning up your directories¶
In order to save disk space, relion has an option to clean up job directories. There are two modes of cleaning: ‘gentle’ cleaning will only delete intermediate files from the job directory being cleaned; ‘harsh’ cleaning also deletes files that may be necessary to launch a new job that needs input from the job being cleaned. For example, harsh cleaning will remove averaged micrographs from a MotionCorr job, or extracted particles stacks from a Particle extraction job, while gentle cleaning will remove all files from itermediate iterations of 2D classification, 3D classification or 3D auto-refine jobs. You can clean individual jobs from the button; or you can clean all jobs from the ‘Jobs’ pull-down menu at the top of the GUI. We used the ‘Gently clean all jobs’ option from that menu before making a tarball of the project directory that we distributed as our precalculated results. You might want to gently clean your project directory before you put your data in long-term storage.
Asking questions and citing us¶
That’s it! Hopefully you enjoyed this tutorial and found it useful. If you have any questions about relion, please first check the FAQ on the relion Wiki and the CCPEM mailing list. If that doesn’t help, use the CCPEM list for asking your question.
If relion turns out to be useful in your research, please do cite our papers and tell your colleagues about it.
The theory behind the refinement procedure in relion is described in detail in:
S.H.W. Scheres (2012) “relion: Implementation of a Bayesian approach to cryo-EM structure determination” J. Struc. Biol., 180, 519-530.
S.H.W. Scheres (2012) “A Bayesian view on cryo-EM structure determination” J. Mol. Biol., 415, 406-418.
A comprehensive overview of how to use relion for all types of classifications is described in:
S.H.W. Scheres (2016) “Processing of structurally heterogeneous cryo-EM data in relion” Meth. Enzym., 579, 125-157.
This tutorial does not cover multi-body refinement, which is useful to describe continuous motions in relatively large complexes. You can find a manuscript with specific instructions on how to perform multi-body refinement on the RELION Wiki