Masks & Postprocessing¶
In this tutorial we have used custom masks, based on geometrical shapes, created externally using tools like dynamo_mask_GUI from dynamo package. For general mask creation, refer to SPA tutorial mask description.
The procedure to sharpen a 3D reference map and estimate the gold-standard FSC curves for subtomogram averaging is strictly the same as described in the SPA tutorial.
Note in the I/O tab an optimisation set file can also be used as input. In that case, an output optimisation set
PostProcess/jobXXX/postprocess_optimisation_set.star is also created, where the reference FSC file has been updated. Thi is useful to be used as input for Tomo CTF refinement and Tomo frame alignment jobs.