Subtomogram tutorial

• Introduction
• Preprocessing
  • Getting organised
• Import tomograms
• Import coordinates
• Make pseudo-subtomograms
  • Running the job
• De novo 3D model generation
  • Running the job
  • Analysing the results
• Initial 3D refinement
  • Running the auto-refine job
  • Analysing the results
• Reconstruct particle
  • Running the job
  • Analysing the results
• High-resolution 3D refinement
  • Pseudo-subtomograms at bin 2
  • Running the auto-refine job at bin 2
  • Pseudo-subtomograms and reference map at bin 1
  • Running the auto-refine job at bin 1
• Masks & Postprocessing
  • Masks
  • Post-processing
• 3D classification
• Tomo CTF refinement
  • Reference map at bin 1 and FSC data
  • Running the job
  • Analysing the results
• Tomo frame alignment
  • Running the job
  • Analysing the results
  • Tomo refinement cycle