The custom data types used in tomography programs in relion are:
These are the most relevant tomography-related programs in relion:
These programs use an existing particle set and a pair of high-resolution 3D reference maps to estimate or improve different properties:
relion_tomo_align : aligns the images of a tilt series and estimates beam-induced motion to better fit a set of particles.
relion_tomo_refine_ctf : refines the astigmatic defocus, signal intensity (i.e. ice thickness) and higher-order aberrations.
relion_tomo_local_particle_refine : offers a rapid way to refine the angles and positions of individual particles.
relion_tomo_fit_blobs_3d : fits a set of spherical manifolds representing vesicles to the observed membranes.
relion_tomo_import_tomograms : imports new tilt series to create or extend a tomogram set.
relion_tomo_import_particles : imports new particles.
relion_tomo_reconstruct_tomogram : creates a (typically low-magnification) tomogram.
relion_tomo_make_reference : runs
relion_postprocesson a pair of reference half maps.
relion_tomo_find_fiducials : detects the fiducial markers in a given data set.